The adaptation of crop plants to environmental stress conditions:
Environmental stress due to drought and salinity are the most serious factors limiting the productivity of agricultural crops, which are predominantly sensitive to low soil moisture and the presence of high concentrations of salts in the soil. The relevance of solutions to these problems is of obvious importance for world’s agriculture in general and the State of California in particular. Crop production in both the Imperial Valley and the San Joaquin Valley is severely affected by limiting water resources and the progressive salinization of the soil. In addition, water resources are becoming increasingly scarce and water quality is decreasing, thus increasing the severity of the problem. Our work demonstrated that transgenic plants expressing IPT (isopentenyltransferase), a gene encoding an enzyme mediating cytokinin (CK) synthesis, under the control of the senescence- and stress-induced SARK promoter were able to survive drought stress with a significant yield advantage over wild-type plants. Moreover, the PSARK::IPT transgenic plants displayed reduced yield penalty and improved grain quality. Our working hypothesis, based on recent findings in our laboratory, is that in addition to the induction of protective mechanisms against the deleterious effects of stress, the partitioning of assimilates and nutrients between source and sink tissues is a key factor in the adaptation of crop plants to adverse environmental conditions in general and water deficit in particular. We have identified a number of genes that regulate hormone homeostasis, alter starch metabolism, and modify the plant source/sink balance during stress. We are characterizing the effect of these genes in ameliorating the effects of stress in the plants and pyramiding genes with complementary function. We use a System Biology approach combining molecular biology, plant transformation, genomics, proteomics and metabolomics to assess phenotype and gene function. We focus our work on cereals, using rice. Millet, wheat and the cereal model plant Brachypodium. Our work has generated a number of patents that have been licensed by the California biotechnology industry to develop salt tolerant and water use efficient cultivars.
The biochemical and molecular basis of fruit ripening:
We are applying a systems biology approach (that combines genomics, metabolomics, proteomics, biochemistry, pre- and post-harvest physiology) to identify ethylene-mediated changes during fruit maturation, ripening and senescence aiming at the identification of key molecular and biochemical determinants that could be manipulated for the development of cultivars with enhanced quality traits. In particular, we are characterizing the metabolite profiles of climacteric and non-climacteric plum fruits and the protein/enzymes associated with ripening processes in climacteric and non-climateric plum fruits. We are analyzing the expression of genes associated with the development of quality traits in climacteric (and non-climacteric fruits) and identifying metabolic/enzymatic pathways associated with pre- and post-harvest quality traits.
Role of ion and pH homeostasis in plant growth and stress responses:
Our general is the characterization and identification of the biochemical and biophysical processes that regulate cell growth and cell expansion. We focus on the regulation of the transport of solutes (K+ and Na+ in particular), the establishment of ion and pH homeostasis in the plant cell and their impact on the regulation of cell volume and the traffic of membrane and their protein cargo between the different cell compartments, and the cell response to environmental changes. Our research builds on the work done by our group towards the characterization of the roles of the intracellular NHX-type of transporters. We have shown the paramount roles of the endosomal NHX5 and NHX6 in plant growth and development, and the vacuolar NHX1-NHX4 in floral development, cell expansion and ionic regulation. We apply a multidisciplinary approach to establish the functional role(s) of each intracellular NHX, combining physiology, biochemistry, genetics, genomics, vesicular membrane transport, the heterologous gene expression in plants, and use of multiple NHX-knockout lines developed in our laboratory.
Lab: 1201 PRB
Office: 1119 PRB
Department of Plant Sciences – Mail Stop 5
University of California
One Shields Ave, Davis, CA 95616
From Highway 80: take Highway 113 north and exit on Hutchison Boulevard. Drive east and turn left on Extension Center. Parking is available for $6 in VP 30. The Plant Reproductive Biology Building and the Bowley Plant Sciences Center are south of VP 30 and west of Transportation & Parking Services. There are several one hour parking spots available on the west side of the building.
From Highway 5: take Highway 113 south and exit on Hutchison Boulevard. Drive east and turn left on Extension Center. Parking is available for $6 in VP 30. The Plant Reproductive Biology Building and the Bowley Plant Sciences Center are south of VP 30 and west of Transportation & Parking Services. There are several one hour parking spots available on the west side of the building.
EXPANDING THE BREEDER’S TOOLBOX FOR BREEDER’S TOOLBOX FOR PERENNIAL GRASSES
The project aims at using a systems-based approach to develop new breeding tools for perennial grasses and apply these tools towards the improvement of switchgrass (Panicum virgatum L.). Our objectives are: (1) Accelerate conventional breeding using the fast generation of doubled haploid lines (developing a CENH3-based method in switchgrass); (2) Use the model perennial grass Brachypodium sylvaticum to identify combinations of transgenes that confer tolerance to multiple abiotic stresses; (3) Develop a gene containment system to minimize gene flow from transgenic switchgrass; (4) Create transgenic switchgrass plants containing the best combinations of transgenes identified in objective 2 and the gene containment system from objective 3; (5) Evaluate the best transgenic switchgrass plants from objective 4 in field trials. Click here
John Vogel [DOE Joint Genome Institute, Walnut Creek, CA94598]; Roger Thilmony [USDA-ARS Western Regional Research Center, Albany CA94710]; Christian Tobias [USDA-ARS Western Regional Research Center, Albany CA94710]
THE EFFECTS OF MODIFICATIONS OF SOURCE-SINK RELATIONSHIPS ON CROP STRESS TOLERANCE
Abiotic stress is the primary cause of crop plant yield losses worldwide. Improving yield production and stability under stressful environments is needed to fulfill the food demand of the ever-growing world population, and an estimated increase of 50% in grain yield of major crops swill be needed by 2050. Drought, the most prominent threat to agricultural production worldwide, accelerates leaf senescence, leading to a decrease in canopy size, loss in photosynthesis and reduced yields. We hypothesized that it may be possible to enhance drought tolerance by altering sink/source relationships in the plant by promoting the stress-induced synthesis of cytokinins. The regulated expression of IPT (isopentenyltransferase) under the control of PSARK significantly improved drought tolerance in both laboratory and field conditions. Transgenic plants produced higher yields than wild-type plants in the field and the seeds from PSARK::IPTplants were normal, indicating that the nutritional value of the transgenic seeds was not altered. We used a multidisciplinary approach that combined genomics, proteomics, metabolomics and enzyme function analysis to identify and characterize cellular/biochemical components that regulate Carbon and Nitrogen metabolism in plants grown under water deficient conditions. Stress-induced cytokinin production had a positive effect on nitrate uptake as well as on the expression of genes associated with primary N assimilation and N re-assimilation, enhanced higher protein synthesis and the strengthening of the transgenic plants sink capacity. A System Biology approach was applied to identify genes and gene networks mediating the stress-response of crops to abiotic stress. A number of genes have been identified, and their expression has been modified in a number of crop species. The role of these genes and their effects on survival to stress is under study. Click here Click here
CELLULAR ION HOMEOSTASIS: EMERGING ROLES OF INTRACELLULAR NHX-TYPE Na+/H+ ANTIPORTERS IN PLANT GROWTH AND DEVELOPMENT:
Na+/H+ antiporters (NHX-type) are important regulators of intracellular pH and K+(Na+) homeostasis in plant cells. In Arabidopsis the NHX gene family includes six intracellular Na+/H+ members (AtNX1-6), which localize to either the vacuole (AtNHX1-4), or to trafficking endosomes (AtNHX5-6). Previously we showed that the double knockout nhx1nhx2 had reduced growth rates, smaller cells, shorter hypocotyls in etiolated seedlings and abnormalities in flower development. Measurements of intravacuolar pH and K+ concentrations indicated that nhx1nhx2 vacuoles were more acidic and accumulated less K+.nhx1nhx2 plants also displayed severe sensitivity to added K+ (10mM) but not to Na+, and the addition of 50mM NaCl to the growth medium partially rescued the growth phenotype. These results demonstrated that NHX1 and NHX2 play significant roles in vacuolar K+/H+ exchange and are key determinants in establishing vacuolar K+ homeostasis and raised questions regarding the cellular ‘compatibility’ of K+ ions, the transport of Na+ into the vacuole, the role of both ions in generating the vacuolar turgor needed to drive cell expansion, as well as the cellular response to high salinity. We extended these studies to a larger comparative analysis of additional multiple knockout lines lacking all possible combinations of NHX1, NHX2, NHX3 and NHX4. Triple and tetra knockouts exhibited complex and unique growth phenotypes suggesting that individual NHX isoforms contribute differentially to cell growth. We measured and compared vacuolar pH, K+ and Na+ concentrations, under varying conditions, in different knockout lines with the goal of quantifying the contribution of each vacuolar isoform to cell pH and ion homeostasis. Furthermore, other results showed that the Golgi and trans-Golgi network-localized NHX members, NHX5 and NHX6 are required for vesicular trafficking, cell growth and salt-stress responses. To investigate the roles of NHX5 and NHX6 in maintaining vesicular pH homeostasis, we developed genetically encoded pH sensors and targeted these to the distinct endomembrane compartments, along the secretory pathway. Measurements of endosomal pH, indicated that a gradual acidification of compartments from the ER to the vacuole exists. Using nhx5nhx6 double knockouts, we further investigated the role of intravesicular pH and ion homeostasis on vesicular trafficking and protein processing. Using a combination of approaches including in vivo protein-protein interactions, biochemical characterization of protein complexes, and in vivo endomembrane pH measurements, we provide evidence indicating that pH and/or ion homeostasis, controlled by NHX5 and NHX6, is a requisite for receptor mediated processing of storage proteins and trafficking of storage proteins to protein storage vacuoles. Click here
CELLULAR AND MOLECULAR DETERMINANTS FOR CLIMACTERIC AND NON-CLIMACTERIC BEHAVIOR IN PLUM FRUITS.
Japanese plums represent the most abundant and variable group among tree species and include most of the fresh-market plums commercialized worldwide. We characterized and compared two Japanese plum cultivars, “Santa Rosa” (SR) and its bud-sport mutant “Sweet Miriam” (SM). These cultivars share the same genetic background but display contrasting ripening behaviors (SR, climacteric and SM, non-climacteric). Both cultivars differ in their sugar metabolism conferring the SM fruits with unusual quality properties (lower glucose and fructose, higher sorbitol and galactose-metabolism related sugars, etc). The main objective of this research is to characterize the differences in the complex sugar metabolic pathways between the cultivars and their possible crosstalk with ethylene biosynthesis-related enzymes, underlying their climacteric and non-climacteric fruit ripening behaviors. Fruits from each each cultivar were harvested at an early (S2: pit hardening) and late (S4: fully-ripe) stages of fruit development and assessed using a Systems Biology approach. Transcriptomics, proteomics and metabolomics methodologies, together with targeted gene expression and enzymatic activity assays were analyzed to reveal complex sugar metabolic interrelations and identify differences between the cultivars that could be associated to the observed changes in sugar homeostasis as well as ethylene biosynthesis and ethylene signaling. This experimental system provides a unique tool to study metabolic pathways underlying climacteric and non-climacteric fruit ripening behaviors and offers several practical applications. Understanding mechanisms that allow fruits to ‘switch’ to a sorbitol-based metabolism would have a great industry impact, since sorbitol is an alternative and healthier natural sweetener to sucrose. In addition, it could also allow the identification of candidate genes for breeding programs focused on fruit quality improvement.
DEVELOPMENT OF ABIOTIC STRESS TOLERANT MILLET FOR AFRICA AND SOUTH ASIA
The goal of this project is to increase and stabilize yields of millet in South Asia and Africa and thus increase the incomes of small farmers in face of the harsh climatic conditions in which pearl millet is usually grown, while also preparing the crop to the even harsher conditions of future climates. By the end of the grant period, we expect to have one year of field trials at two locations in India to evaluate an increase in yield. The project will generate a range of products, either transgenic lines with triple construct, with or without a terminal drought tolerance QTL, and individual isolines containing several QTLs for components of terminal drought adaptation, all in a similar genetic background and ready for pyramiding. Our development objective is to demonstrate the value of these products to a point where the private sector will invest further and where a path for public sector delivery becomes feasible.
Vincent Vadez [ICRISAT, International Crops Research Institute for the Semi-Arid Tropics, Patancheru 502 324, Andhra Pradesh, India]
Click on any paper to download or link to Pubmed
- Yang Zeng, Qing Li, Haiya Wang, Jianliang Zhang, Jia Du, Huimin Feng, Eduardo Blumwald, Ling Yu, and Guohua Xu (2017) Two NHX-type transporters from Helianthus tuberosus improve the tolerance of rice to salinity and nutrient deﬁciency stress. Plant Biotechnology Journal (2017) pp. 1–12 doi:10.1111/pbi.12773
- Macarena Farcuh1, Bosheng Li1, Rosa M. Rivero2, Lyudmila Shlizerman3, Avi Sadka3 and Eduardo Blumwald (2017). Sugar metabolism reprogramming in a non-climacteric bud mutant of a climacteric plum fruit during development on the tree. Journal of Experimental Botany, Vol. 68, No. 21–22 pp. 5813–5828, 2017 doi:10.1093/jxb/erx391
- Sade N, Ummajkitikorn K, Rubio Wilhelmi MM, Wright M, Wang S, Blumwald, E (2017). Delaying chloroplast turnover increases water-deficit stress tolerance through the enhancement of nitrogen assimilation in rice. J. Experimental. Botany. doi:10.1093/jxb/erx247
- Sade N, Rubio Wilhelmi MM, Ummajkitikorn K, Blumwald, E (2017). Stress-induced senescence and plant stress tolerance. J. Experimental. Botany. doi:10.1093/jxb/erx235.
- San Martin A, Perez-Diaz R, Soto F, Madrid-Espinoza J, Gonzalez-Villanueva E, Pizarro L, Norambuena L, Tapia J, Tajima H, Blumwald E, Ruiz-Lara S (2017). Involvement of SchRabGDI1 from Solanum chilense in endocytic trafficking and tolerance to salt stress. Plant Science. 263:1-11
- Decima Oneto C, Bossio E, Faccio P, Beznec A, Blumwald E, Lewi D. (2017). Validation of housekeeping genes for qPCR in maize during water deficit stress conditions at flowering time. Maydica 62.2 – M13.
- Shaar-Moshe L, Blumwald E, Peleg Z (2017). Unique Physiological and Transcriptional Shifts under Combinations of drought, heat and salinity. Plant Physiology 174:421-434.
- Yoon S, Aucar S, Hernlem BJ, Edme S, Palmer N, Sarath G, Mitchell R, Blumwald E, Tobias CM (2017). Generation of Octaploid Switchgrass by Seedling Treatment with Mitotic Inhibitors. BioEnergy Res. 10:344-352.
- Choudhury FK, Rivero RM, Blumwald E, Mittler R (2017). Reactive oxygen species, abiotic stress and stress combination. Plant Journal 90:856-867.
- Saha P, Sade N, Arzani A, Rubio Wilhelmi MM, Coe KM, Li B, Blumwald E (2016). Effects of abiotic stress on physiological plasticity and water use of Setaria viridis L. Plant Science 251:128-138.
- Saha P, Blumwald E (2016). Spike-dip transformation of Setaria viridis. Plant Journal 86:89-101.saha et al.plant science.2016
- Oneto, C. D., Otegui, M. E., Baroli, I., Beznec, A., Faccio, P., Bossio, E., Blumwald E, Lewi D. (2016). Water deficit stress tolerance in maize conferred by expression of an isopentenyltransferase (IPT) gene driven by a stress-and maturation-induced promoter. J. Biotechnol. 220:66-77.
- Bascuñán-Godoy, L., Reguera, M., Abdel-Tawab, Y. M., & Blumwald, E. (2016). Water deficit stress-induced changes in carbon and nitrogen partitioning in Chenopodium quinoa Willd. Planta 243:591-603.
- Shalom L, Shlizerman L, Zur N, Doron-Faigenboim A, Blumwald, E, Saka A (2015).Molecular characterization of SQUAMOSA PROMTOER BINDING PROTEIN-LIKE (SPL) gene family from citrus and the effect of fruit load on their expression. Front Plant Sci. 6:389.
- Tamaki H, Reguera M, Abdel-Tawab YM, Takebayashi Y, Kasahara H, Blumwald E (2015). Targeting hormone-related pathways to improve grain yield in rice: a chemical approach. PloS ONE 10(6):e0131213.
- Kim H-Y, Saha P, Farcuh M, Li B, Sadka A, Blumwald, E (2015). RNA-seq analysis ofspatiotemporal gene expression patterns during plum fruit development reveals candidate genes for transcript normalization using quantitative Real-Time PCR. Plant Mol. Biol. Rep. 33:1634-1649.
- Raineri J, Wang S, Peleg Z, Blumwald E, Chan RL (2015). The rice transcription factor OsWRKY47 is a positive regulator of the response to water stress deficit. Plant Mol. Biol. 88:401-413.
- Reguera M, Bassil E, Tajima H, Wimmer M, Chanoca L, Otegui M, Paris N, Blumwald E(2015). pH regulation by NHX-type antiporters is required for receptor mediated protein trafficking to the vacuole. Plant Cell 27:1200-1217.
- Wang S, Blumwald E (2015). The regulation of stress-induced chloroplast degradation via a process independent of autophagy and senescence-associated vacuoles. Plant Cell 26(12):4875-88. doi: 10.1105/tpc.114.133116.
- Shen G, Wei J, Qiu X, Hu R, Kuppu S, Auld D, Blumwald E, Gaxiola R, Payton P, Zhang H (2015). Co-overexpression of AVP1 and AtNHX1 in Cotton Further Improves Drought and Salt Tolerance in Transgenic Cotton Plants. Plant Mol. Biol. Rep.33:167-177.
- Mittler R, Blumwald E (2015). The roles of ROS and ABA in systemic acquired acclimation. Plant Cell. 27:64-70.
- Conde A, Regalado A, Rodrigues D, Costa MJ, Blumwald E, Chaves MM, Geros H (2015). Polyols in grape berry – transport and metabolic adjustments as a physiological strategy for water stress tolerance in grapevine. Journal Experimental Botany. 66(3):889-906.
- Kim HY, Farcuh M, Cohen Y, Crisosto C, Sadka A, Blumwald E (2015). Non-climacteric ripening and sorbitol homeostasis in plum fruits. Plant Science 231:30-39.
- Martins V, Teixeira A, Bassil E, Hanana M, Blumwald E, Geros H (2014). Copper-based fungicide Bordeaux mixture regulates the expression of Vitis vinifera copper transporters (VvCTrs). Aust. J. Grape and Wine Res. 20:451-458.
- Bassil E and Blumwald E (2014) The ins and outs of intracellular ion homeostasis: NHX-type Cation/H+ transporters. Current Opinion in Plant Biology. 22:1-6.
- Saha P and Blumwald E (2014) Assessing reference genes for accurate transcript normalization using quantitative Real-Time PCR in Pearl Millet [Pennisetum glaucum (L.) R. Br. PloS ONE. 9(8) e106308 doi:10.1371/journal.pone.0106308.
- McCubbin T, Bassil E, Zhang S, Blumwald E (2014). Vacuolar Na+/H+ NHX-type antiporters are required for cellular K+ homeostasis, microtubule organization and directional root growth. Plants 3:409-426.
- Martins, V, Texeira A, Bassil E, Hanana M, Blumwald E, Geros H (2014). Metabolic changes of Vitis vinifera berries and leaves exposed to Bordeaux mixture. Plant Physiol and Biochem. 82:270-278.
- Martins V, Bassil E, Hanana M, Blumwald E, Gerós H (2014) Copper homeostasis in grapevine: Functional characterization of the Vitis vinifera copper transporter Planta 240: 3029-3044.
- Shalom L, Zur N, Faigenboim A, Halak S, Blumwald E, Sadka A (2014). Fruit load induces changes in global gene expression and in ABA and IAA homeostasis in citrus buds. J. Experimental Botany 65: 3029-3044.
- Suzuki N, Rivero RM, Shulaev V, Blumwald E, Mittler R (2014). Abiotic and Biotic Stress Combinations. New Phytologist, 203:32-43.
- Gimeno J, Eattock N, Van Deynze A, Blumwald E (2014). Selection and validation of reference genes in switchgrass (Panicum virgatum) using quantitative real-time RT-PCR. PLoS ONE 9(3):e91474.(doi:10.1371/journal.pone.0091474)
- Reguera M, Bassil ES, Blumwald E (2014). Intracellular NHX-type Cation/H+ Antiporters in Plants. Molecular Plant, 7:261-263
- Rubio-Wilhelmi MM, Reguera M, Rujiz, JM, Blumwald E. (2014). PSARK::IPT expression. causes protection of photosynthesis in tobacco plants during N-defficiency. Env. Exp. Bot. 98:40-46.
- Reguera M, Peleg Z, Abdel-Tawab YM, Tumimbang EB, Delatorre CA, Blumwald E (2013). Stress-Induced CK Synthesis Increases Drought Tolerance through the Coordinated Regulation of Carbon and Nitrogen Assimilation in Rice. Plant Physiol. 163:1609-1622.
- Martiniere A, Bassil E, Jublanc E, Alcon C, Reguera M, Sentenac H, Blumwald E, Paris N (2013). In vivo intracellular pH measurements reveal an unexpected pH gradient in the plant endomembrane system. Plant Cell, 25:4028-4043.
- Kuppu S, Mishra N, Hu R, Sun L, Zhu X, Shen G, Blumwald E, Payton P, Zhang H (2013). Water-deficit inducible expression of a cytokinin biosynthetic gene IPT improves drought tolerance in cotton. PLoS ONE, 8(5):e64190.
- Goldberg-Moeller R, Shalom L, Shlizerman L, Samuels S, Zur N, Ophir R, Blumwald E, Sadka A. (2013) Effects of gibberellin treatment during flowering induction period on global gene expression and the transcription of flowering-control genes in Citrus buds. Plant Science. 198:46-67.
- Dutta I, Martin KP, Tumimbang E, Tajima H, Zaid A, Blumwald E. (2013). Sonication-assisted efficient Agrobacterium-mediated genetic transformation of the multipurpose woody shrub Leptadenia pyrotechnica. Plant Cell Tissue & Organ Culture. 12:289-301.
- Arif A, Zafar Y, Arif M, Blumwald E. (2013). Improved growth, drought tolerance and ultrastructural evidence of increased turgidity in tobacco plants overexpressing Arabidopsis vacuolar pyrophosphatase (AVP1). Mol. Biotechnol. 54:379-392.
- Shalom L, Samuels S, Zur N, Shlizerman L, Zemach H, Ofir R, Blumwald E, Sadka A. (2012). Alternate bearing in citrus: Changes in the expression of flowering control genes and in global gene expression in ON- versus OFF-crop trees. PLoS ONE, 7(10):e46930.
- Bassil ES, Coku A, Blumwald E. (2012). Cellular ion homeostasis emerging roles of intracellular NHX Na+/H+ antiporters in plant growth and development. J. Exp. Bot. 63:5727-5740
- Rubio-Wihelmi M, Sanchez-Rodriguez E, Leyva R, Blasco B, Romero L, Blumwald E, Ruiz JM. (2012) Response of carbon and nitrogen-rich metabolites to nitrogen deficiency in PSARK-IPT tobacco plants. Plant Physiol. Biochem. 57:231-237.
- Delatorre CA, Cohen Y, Liu L, Peleg Z, Blumwald E. (2012). The regulation of the SARK Promoter activity by hormones and environmental signals. Plant Sci. 193:39-47.
- Rubio-Wilhelmi MM, Sanchez-Rodriguez E, Blasco B, Rios, J, Romero L, Blumwald E, Ruiz JM (2012). Ammonium formation and assimilation in PSARK::IPT tobacco transgenic plants under low N. J. of Plant Physiol. 169: 157-162
- Reguera M, Peleg Z, Blumwald, E. (2012). Targeting metabolic pathways for genetic engineering abiotic stress in crops. Biochem. Biophys. Acta.1819:186-194.
- Qin H, Gu A, Zhang J, Sum L, Kuppu S, Zhang Y, Burow M, Payton P, Blumwald E, Zhang H (2011). Regulated expression of an isopentenyltransferase gene (IPT) in peanut significantly improves drought tolerance and increases yield under field conditions. Plant Cell Physiol. 52:19045-1914.
- Rubio-Wilhelmi MM, Sanchez-Rodriguez E, Rosales MA, Blasco B, Rios, J, Romero L, Blumwald E, Ruiz JM (2011). Cytokinin-dependent improvement in transgenic PSARK::IPT tobacco under nitrogen deficiency.J.Agr.Food Chem. 59: 10491-10495.
- Bassil E, Tajima H, Liang Y-C, Ohto M, Ushijima K, Nakano R, Esumi T, Coku A, Blumwald E (2011). The Arabidopsis Na+/H+ Antiporters NHX1 and NHX2 Regulate Vacuolar pH and K+ Homeostasis to Control Growth Flower Development and Reproduction Plant Cell 23: 3482-3511.
- Reguera M, Peleg Z, Blumwald, E. (2011). Targeting metabolic pathways for genetic engineering abiotic stress in crops. Biochim. Biophys. Acta.DOI:10.1016/j.bbagrm.2011.08.005.
- Katz, E; ,Boo, K-H; Kim HY, Eigenheer, RA, Phinney, BS; Shulaev, V; Negre-Zakharov, V; Sadka, A; Blumwald, E. (2011) Label-free shotgun proteomics and metabolite analysis reveal a significant metabolic shift during Citrus fruit development. J. Exp. Bot. doi: 10.1093/jxb/err197.
- Sorkina, A; Bardosh, G; Liu, Y-Z; Fridman, I; Schlizerman, L; Zur, N; Or, E; Goldschmidt, E, Blumwald, E, Sadka, E (2011). Isolation of a citrus promoter specific for reproductive organs and its functional analysis in isolated juice sacs and tomato. Plant Cell Rep. . DOI:10.1007/s00299-011-1073-3.
- Degu, A; Hatew, B; Nunes-Nesi, A; Schlizerman, L; Zur, N; Katz, E; Fernie, AR; Blumwald, E; Sadka, A. (2011). Inhibition of aconitase in citrus fruit callus results in a metabolic shift towards amino acid synthesis. Planta DOI:10.1007/s00425-011-1411-2.
- Hanana M, Lamia H, Cagnac O, Blumwald, E (2011) Cellular mechanisms and strategies of salinity tolerance in plants. Env. Rev. 19: 121-140.
- Rubio-Wilhelmi MM, Sanchez-Rodriguez E, Rosales MA, Blasco, Rios JJ, Romero L, Blumwald E, Ruiz JM (2010). Effect of Cytokinins on Oxidative Stress in Tobacco Plants Under Nitrogen Deficiency. Env. Exp. Bot. 72:167-173.
- Peleg, Z, Reguera, M., Walia, H., Blumwald, E. (2011) Cytokinin mediated source-sink modifications improve drought tolerance and increases grain yield in rice under water stress, Plant Biotechnol. J. 9:747-758.
- Peleg Z, Apse MP, Blumwald, E (2011). Engineering Salinity and Water Stress Tolerance in Crop Plants: Getting closer to the field. Adv. Bot. Res.57:405-443.
- Bassil E., Ohto, M., Esumi, T., Tajima, H., Zhu Z., Belmonte, M., Peleg, Z., Yamaguchi, T.,Blumwald, E. (2011). The Arabidopsis intracellular Na+/H+ antiporters AtNHX5 and AtNHX6 are implicated in novel endosomal functions associated with plant growth and development. Plant Cell. 23:224-239.
- Peleg, Z and Blumwald, E. (2011). Hormone homeostasis and abiotic stress tolerance in crop plants. Curr. Op. Plant Biol. 14:1-6.
- Katz E, Fon MG, Eigenheer RA, Phinney BS, Fass, JN, Lin, D, Sadka A., Blumwald, E (2011). A label-free differential quantitative mass spectrometry method for the characterization and identification of protein changes during citrus fruit development. Proteome Sci. 8:68.
- Pasapula V, Shen G, Kuppu S, Paez-Valencia J, Mendoza M, Hou P, Chen J, Auld D, Blumwald, E, Zhang H, Gaxiola R and Payton P (2011). Expression of an Arabidopsis vacuolar H+-pyrophosphatase gene (AVP1) in cotton improves drought-and salt-tolerance and increases fiber yield in the field conditions. Plant Biotechnol. J. 9: 88-99.
- Rivero RM, Walia H, Blumwald E (2010). Enhanced cytokinin synthesis in tobacco plants expressing PSARK::IPT prevents the degradation of photosynthetic protein complexes during drought. PCP 51(11):1929-41.
- Thomson, MJ; de Ocampo M;, Egdane J: Rahman, MA; Sajise, A;,Adorada D; Tuminbang, E; Blumwald, E; Seraj, Z;, Singh, RK; Gregorio, GB, Ismail, AM. (2010) Characterization of Saltol quantitative trait locus for salinity tolerance in rice. Rice, 3:148-160.
- Xu K, Blumwald E, Xia T (2010). A novel plant vacuolar Na+/H+ antiporter gene evolved by DNA shuffling confers improved salt tolerance in yeast. J. Biol. Chem.285:22999-23006.
- Adler G, Blumwald E, Bar-Zvi D. (2010). The sugar beet gene encoding the sodium/proton exchanger 1 (BvNHX1) is regulated by a MYB transcription factor. Planta. 232:187-195.
- Mittler R, Blumwald E. (2010). Genetic engineering for modern agriculture: Challenges and Perspectives. Annu. Rev. Plant Biol. 61:443-462.
- Pasapula V, Shen G, Kuppu S, Paez-Valencia J, Mendoza M, Hou P, Chen J, Auld D, Blumwald, E, Zhang H, Gaxiola R and Payton P (2010). Expression of an Arabidopsis vacuolar H+-pyrophosphatase gene (AVP1) in cotton improves drought- and salt-tolerance and increases fiber yield in the field conditions. Plant Biotechnology Journal .pp 1-12
- Rivero RM, Shulaev V, Blumwald, E. (2009) Cytokinin-dependent photorespiration and the protection of photosynthesis during water deficit. Plant Physiology 150:1380-1393.
- Barney, JN., Mann, J., Kyser, GB., Blumwald, E., Van Deynze, A., DiTomaso, JM. (2009). Tolerance to extreme soil moisture stress: ecological implications. In press, Plant Science.
- Hanana, M., Cagnac, O., Ahmed, M., Blumwald, E. (2009). Topological model of a grapevine vacuolar NHX antiporter structure. Botany, 87: 339-347.
- Rahman, S., Ohto, M., Seraj, Z.I., Blumwald, E. (2007). Bacteriophage Lambda genomic library construction of Oryza sativa L. var. Pokkali. Plant Tissue Cult. & Biotech. 17: 149-159.
- Rivero, RM., Kojima, M., Gepstein A., Sakakibara, H., Mittler, R., Gepstein, S., and Blumwald, E. (2007). Delayed leaf senescence induces extreme drought tolerance in a flowering plant. Proc. Natl. Acad. Sci. USA 104:19631-19636.
- He, C; Shen, G; Paspula, V., Luo, J., Venkataramani, S., Qiu, X., Kupu, S, Kryeyevi, A., Holaday, S., Auld, D., Blumwald, E.,, and Zhang, H. (2007). Ectopic expression of AtNHX1 in cotton (Gossypium Hirsutum) increases proline content and enhances photosynthesis under salt stress conditions. J. Cotton Sci. 11:266-274.
- Cagnac, O., Leterrier, M., Yeager, M. and Blumwald, E. (2007). Identification and characterization of vnx1p, a novel type of vacuolar monovalent cation/H+ antiporter of Saccharomyces cerevisiae. J. Biol. Chem. 282: 24284-24293.
- Katz., E., Fon, M., Lee, Y.J., Phinney, B.S., Sadka, A, and Blumwald, E. (2007). The citrus fruit proteome: insights into citrus fruit metabolism. Planta 226: 989-1005.
- Shlizerman, L; Marsh, K; Blumwald, E & Sadka, A.(2007) Iron limitation induces citric acid accumulation and reduces activity of the cytosolic aconitase in citrus. Physiol. Plant. 131:72-79.
- Hanana, M., Cagnac, O., Yamaguchi, T., Said, H., Ghorbel, A., Blumwald, E. (2007). A grape berry (Vitis vinifera L.) cation/proton antiporter is associated with berry ripening. Plant Cell Physiol. 48:804-811.
- Apse, M.P. & Blumwald, E. (2007). Na+ transport in plants. FEBS Lett. 581:2247-2254.
- Eingenheer, R.A., Lee, Y.K., Blumwald, E. Phinney, B.S. & Gelli, A. (2007). Extracellular glycosylphosphatidylinositol-anchored mannoproteins and proteases of Cryptococcus neoformans. FEMS Yeast Res. 7: 499-510.
- Alpi, A., Amrhein, N., Bertl, A., Blatt, M.R., Blumwald, E., Cervone, F., Dainty, J., et al. (2007). Plant neurology: no brain, no gain? Trends Plant Sci. 12: 135-136.
- Sottosanto, J.B. , Saranga, Y., Blumwald, E. (2007). Impact of AtNHX1, a vacuolar Na+/H+ antiporter, upon gene expression during short and long term salt stress in Arabidopsis thaliana. BMC Plant Biology. 7: 18. (doi:10.1186/1471-2229-7-18).
- Novak, J.P. Kim, S-Y, Xu, J., Modlich, O., Volsky, D.J., Honys, D., Slonczewski, J.L., Bell, D.A., Blattner, F.R., Blumwald, E., Boerma, M., Cosio, M., Gatalica, M., Hajduch, M., Hidalgo, J., McInnes, R.R., Miller III, M.C., Penkowa, M., Rolph, M.S. Sottosanto, J.B., St-Arnaud, R., Szego, M.J., Twell D., Wang, C. (2006). Generalization of DNA microarray dispersion properties: microarray equivalent of t-Distribution. Biology Direct 1:27 doi:10.1186/1745-6150-1-27
- Sahi, C., Singh, A., Kumar, K., Blumwald, E. & Grover, A. (2006). Salt stress response in rice: genetics, molecular biology and comparative genomics. Functional & Integrative Genomics 6: 263-284.
- Shimada, T., Nakano, R., Shulaev, V., Sadka, A. and Blumwald, E. (2006). Vacuolar citrate/H+ symporter of citrus juice cells. Planta 224:472-480.
- Sahi, C., Singh, A., Blumwald, E. & Grover, A. (2006). Beyond osmolytes and transporters: novel plant stress tolerance-related genes from transcriptional profiling data. Physiol. Plant. 127:1-9.
- Yamaguchi, T and Blumwald, E. (2005). Developing salt tolerant crop plants: Challenges and Opportunities. Trend Plant Sci. 12:615-620.
- He, C., Yan, J., Shen, G., Fu, L., Holaday, AS., Auld, D., Blumwald, E. & Zhang, H. (2005). Expression of an Arabidopsis vacuolar sodium/proton antiporter gene in cotton improves photosynthetic performance under salt conditions and increases fiber yield in the field. Plant Cell Physiol. 46:1848-1854.
- Yamaguchi, T., Aharon, G.S., Sottosanto, J.B. & Blumwald, E. (2005). Vacuolar Na+/H+ antiporter cation selectivity is regulated by calmodulin from within the vacuole in a Ca2+- and pH-dependent manner. Proc. Natl. Acad. Sci USA. 102:16107-16112.
- Sottosanto, J.B., Gelli, A., Blumwald, E. (2004). DNA array analyses of Arabidopsis thaliana lacking a vacuolar Na+/H+ antiporter: Impact of AtNHX1 on gene expression. Plant J. 40:752-771.
- Hong, Z., Bednarek, S.Y., Blumwald, E., Hwang, I., Jurgens, G., Menzel, D. Osteryoung, K.W., Raikhel, N.V., Shinozaki, K., Tsutsumi, N., Verma, D.P.S. (2004) A unified nomenclature for Arabidopsis dynamin-related large GTPases based on homology and possible functions. Plant Mol. Biol. 53:261-265.
- Yamaguchi, T., Apse, MP. Apse, Shi, H., Blumwald, E. (2003). Topological analysis of a plant vacuolar Na+/H+ antiporter reveals a luminal C-terminus that regulates the antiporter cation selectivity. Proc. Natl. Acad. Sci USA. 100:12510-12515.
- Apse, M.P., Sottosanto, J. & Blumwald, E. (2003). Ion homeostasis in Arabiodpsis thaliana: role of the vacuolar Na+/H+ antiporters. Plant J. 36:229-239.
- Aharon, G.S., Apse, M.P., Duan, S., Hua, X., Zhang, H-X. & Blumwald, E. (2003) Characterization of a family of vacuolar Na+/H+ antiporters in Arabidopsis thaliana. Plant & Soil 253:245-256.
- Lam, B.C-H & Blumwald, E (2002). Domains as functional building blocks of plant proteins. Trends Plant Sci. 7:544-549.
- Xia, T, Apse, MP, Aharon, GS. & Blumwald, E. (2002). Identification and characterization of a NaCl-inducible vacuolar Na+/H+ antiport in Beta vulgaris. Physiol. Plant 116:206-212.
- Lam, B.C-H, Bianchi, F. & Blumwald, E. (2002). Regulation of ADL6 activity by its associated molecular network. Plant J. 31:565-576.
- Ruiz, J.M. & Blumwald, E. (2002). Salinity-induced glutathione synthesis in Brassica napus. Planta 214: 965-969.
- Apse, MP, & Blumwald, E. (2002). Engineering salt tolerance in plants. Curr. Op. Biotechnol. 13:146-150.
- Lam, B.C-H, Sage, T.L., Bianchi, F. & Blumwald, E. (2001). Role of SH3 domain- containing proteins in clathrin-mediated endocytosis in Arabidopsis thaliana. Plant Cell 13:2499-2512.
- Zhang, H-X. Hodson, J., Williams, J.P. & Blumwald, E. (2001). Engineering salt- tolerant Brassica Plants: Characterization of yield and seed oil quality in transgenic plants with increased vacuolar sodium accumulation.. Proc. Natl. Acad. Sci. USA 98:12832-12836.
- Zhang, H-X. & Blumwald, E. (2001). Transgenic salt tolerant tomato plants accumulate salt in the foliage but not in the fruits. Nature Biotechnology 19:765-768.
- Palma, D.A., Blumwald, E. & Plaxton, W.C. (2000). Upregulation of vacuolar H+- translocating pyrophosphatase by phosphate starvation of Brassica napus (rapessed) suspension cultures. FEBS Lett. 486:155-158.
- Snedden, W.A. & Blumwald, E. (2000). A calmodulin-binding diacylglycerol kinase from tomato is produced by alternative splicing at its calmodulin-binding domain. Plant J. 24:317-326.
- Blumwald, E. (2000) Salt transport and salt resistance in plants and other organisms. Curr Op Cell Biol 12:431-434.
- Marshall, J.G., Beardmore, T., Whittle, C.A., Wang, B., Rutledge, R.G. & Blumwald, E. (2000). Delay of germination of silver maple (Acer sacharinum. L seeds by paclobutrazol. Can J For Res. 30: 557-565.
- Blumwald, E., Aharon, G.S. & Apse, P. (2000) Na+ transport in plant cells. Biochim. Biophys. Acta 1465:140-151.
- Apse, M.P., Aharon, G.S., Snedden, W.A. & Blumwald, E. (1999). Overexpression of a vacuolar Na+/H+ antiport confers salt tolerance in Arabidopsis. Science 285:1256-1258.
- Marshall, J.G., Dumbroff, E.B., Thatcher, B.J., Martin, B., Rutledge, R.G. & Blumwald, E.(1999). Synthesis and insolubilization of cell wall proteins during osmotic stress. Planta 208:401-408.
- Glenn, E., Brown, J.J. & Blumwald, E. (1999). Salt-tolerant mechanisms and crop potential of halophytes. Crit. Rev. Plant Sci. 18:227-255.
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IN THE NEWS
By Pat Bailey
A genetically engineered tomato plant that thrives in salty irrigation water and may hold the key to one of agriculture’s greatest dilemmas has been developed by plant biologists at UC Davis and […]
Genetically engineered crop plants that survive droughts and can grow with 70 percent less irrigation water have been developed by an international team led by researchers at the University of California, Davis. The discovery […]
November 9, 2012
Blumwald and his colleagues will use the grants to develop new molecular biology tools to accelerate switchgrass breeding and biotechnology tools to develop new varieties of pearl millet, a small-seeded grass. (Karin […]